The Impacts of TRR14-Overexpression on Arabidopsis thaliana Growth and Photosynthetic Parameters
نویسندگان
چکیده
BACKGROUND TRR14 protein is a small protein, a member of a multigene family in Arabidopsis which was found as the fi rst protein during screening seedlings for their resistant to the trehalose sugar. OBJECTIVES A number of TRR14-overexpressing plants were subjected to the characterization in the present research, among which, the associated morphological features and changes accompany growth pattern and photosynthesis related parameters. MATERIALS AND METHODS TRR14 gene was isolated from Arabidopsis Thaliana and cloned into the pBin-35S vector. Recombinant vector was transferred to the Arabidopsis (Col-0) via Agrobacterium tumefaciens using the Floral Dipping method. Seeds from the TRR14 overexpressed (TRR14) and the Col-0 wild-type (WT) plants were shown on soil under long day conditions. Several measurements were then performed including determination of the fresh and dry weights, leaf area, chlorophyll a and b (Chl a and Chl b) content, Chl a/b ratio, total chlorophyll and carotenoids content, soluble and insoluble sugars content, total and soluble protein content, the Hill reaction rate, chlorophyll fl uorescence, as well as photorespiration rate. Meanwhile, the chloroplastic proteins were investigated by SDS-PAGE analysis. RESULTS TRR14 plants showed a signifi cant increase in fresh and dry weights, leaf area, and total and soluble protein content along with a signifi cant decrease in the insoluble sugar contents was observed in comparison to the WT plants. Chl a, Chl b, total chlorophyll content, Chl a/b ratio, carotenoids content, Hill reaction rate, and chlorophyll fl uorescence didn't show a signifi cant diff erence between TRR14 and WT plants. The SDS-PAGE gel electrophoresis of the chloroplastic proteins showed a thick band with a molecular mass of 25 kDa in TRR14-overexpressed plants, compared to the WT plants. Remarkably, photorespiration rate was decreased in TRR14 plants compared to WT plants. CONCLUSION The increased biomass of TRR14 transformed plants might be due to its ability in reducing photorespiration through concentrating CO2 in the leaf's intercellular spaces.
منابع مشابه
The impacts of TRR14 over-expression on Arabidopsis thaliana growth and some photosynthetic parameters
Background: TRR14 protein is a small member of a multi-gene family in Arabidopsis and is the first ones found during screening of seedlings for their resistant to the trehalose sugar.Objectives: Characterization ofTRR14 over-expressed plants with respect to morphological changes, growth and photosynthesis related parameters.Materials and methods: TRR14gene was isolated from Arabidop...
متن کاملFunctional analysis of glycin rich- RNA binding protein, a suppressor of trehalose-6-phosphate mediating growth arrest in Arabidopsis thaliana
Metabolism of the alpha-1,1 glucose disaccharide, trehalose, is indispensable in plants. In the Murashigeand Skoog (MS) medium, trehalose inhibits plant growth and allocation of carbon to roots. A suppressorof trehalose-6-phosphate (T6P) mediated growth arrest, GR-RBP2, is characterized in more detail.Phylogenetic analysis revealed that GR-RBP2 is a protein of likely prokaryot...
متن کاملمشکلات روشهای موجود و ارائه دو روش جدید کشت هیدروپونیک گیاه آرابیدوپسیس تالیانا
Arabidopsis thaliana is a suitable model plant for genetic and molecular biology studies in higher plants. However, its hydroponic culture for biochemical and physiological studies is a challenge due to small size, capillary roots and little biomass at maturity. Several cultural systems have been suggested for Arabidopsis thaliana hydroponic culture, each having special advantages and disadvant...
متن کاملYeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
SETH4 coding sequence with 2013 bp is a member of gene family expressed in gametophytic tissues of Arabidopsis thaliana. This fragment was PCR amplified using Kod Hi Fi DNA polymerase enzyme. This fragment was cloned into pGBKT7 bate vector and transformed E. coli DH5? cells containing vector were selected on LB medium containing Kanamycin. Finally, pGBKT7-SETH4 bate was transformed into yeast ...
متن کاملMicroarray analysis of gene expression patterns in Arabidopsis seedlings under trehalose, sucrose and sorbitol treatment
Trehalose is the non-reducing alpha-alpha-1, 1-linked glucose disaccharide. The biosynthesisprecursor of trehalose, trehalose-6-phosphate (T6P), is essential for plant development, growth,carbon utilization and alters photosynthetic capacity but its mode of action is not understood. In thecurrent research, 6 days old seedlings of Arabidopsis thaliana (Columbia ecotype) were grown inliquid cultu...
متن کامل